Invitrogen topo ta cloning kit manual

Please note that the kits no longer contain b-mercaptoethanol or stop solution but now contain a salt solution which is added to the cloning reaction. The TOPO vector has an overhanging 3' T. Your PCR product must have an overhanging 3' A. Be sure to include a final extension step of 7 . TOPO ® TA Cloning ® Kits are available with pCR ™ TOPO ® or pCR ™ II-TOPO ® vector. Kits with competent cells are available with One Shot ® Chemically or Electrocomp ™ competent cells as described in the following table (see page. 8: for the genotypes of the strains). Note: Cat. no. is not supplied with competent cells. Select TOPO ® TA Cloning ®File Size: KB. The TOPO“ TA Cloning¤ Kit is shipped on dry ice. Each kit contains a box with TOPO“ TA Cloning¤ reagents (Box 1) and a box with TOP10 or TOP10F«One Shot“ competent cells (Box 2). Store Box 1 at ¡C and Box 2 at ¡C. Types of TOPO“ TA Cloning¤ Kits Two different types of TOPO“ TA Cloning¤ Kits are available in two different.

TOPO ® PCR Cloning Kits are shipped on dry ice. Kits supplied with competent cells containa box with Zero Blunt ® TOPO ® PCR Cloning reagents (Box 1) and a box with One Shot ® E. coli (Box 2). Zero Blunt ® TOPO ® PCR Cloning Kit supplied with the PureLink ® Quick Plasmid Miniprep Kit (Cat. no.K) is shipped with an additional box. Select TOPO ® TA Cloning ® Kits are also available with PureLink ® Quick Plasmid Miniprep Kit. Product Cat. no. One Shot ® Cells Type of Cells Reactions TOPO ® TA Cloning ® Kit (with pCR ™ TOPO ® vector) K TOP chem. competent: K TOP chem. competent: KJ TOP chem. competent: User Manual Corporate Headquarters Invitrogen Corporation Faraday Avenue Carlsbad, CA T: 1 7F: 1 7E: www.doorway.rue@www.doorway.ru For country-specific contact information visit our web site at www.doorway.ru pENTR™ Directional TOPO® Cloning Kits Five-minute, directional TOPO® Cloning of.

www.doorway.ru TOPO TA Cloning® Kits are available with either pCR®TOPO® or included in this kit. Primer. Sequence. pMoles Supplied. Cloning kit contains comp cells, optimized 2X Cloning Master Mix, a ligation enhancer + linearized vector with a novel mechanism for low background. The M13 Forward (−20) and M13 Reverse primers are included to help you sequence your insert. Refer to the maps on page (pCR™TOPO® vector) or page 30 .